Improvement of AAV purification

The standard protocol for purification of AAV vectors contains a time consuming freeze-thaw lysis of the producer cells. The Viral Vector Facility managed to improve this step using the Minilys from Bertin Technologies. With the Minilys, lysis of cells is accomplished within 5 minutes. Infectivity and viral titers are not impaired by using the Minilys.

More detailed information can be found in the application note and the application video.

Application Note - Minilys (PDF, 363 KB)

Melanie Rauch

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