AAV-6(F129L) offers superior transduction efficiency of epithelial cells
In an effort to better understand the biological significance of the six amino acids that differ between the capsids of AAV serotype 1 and 6 (one located in the VP1 the remaining five located in the VP3 subunit), all twelve possible singleton exchanges were produced and studied (see Wu et al, 2006). While the study of Wu et al. mainly focused on the significance of the K531E (AAV-6) or E531K (AAV-1) mutation on the heparin binding (the K531E mutation ablates the heparin binding motif) and transduction efficiency, a subsequent study (see Limberis et al., 2009) identified the F129L mutation in AAV-6 vectors to provide superior transduction efficiency of mouse airway epithelium in vivo and human ciliated airway epithelium in vitro compared to nineteen AAV isolates (including the parental AAV-6). Clearly, AAV-6(F129L) is a valid candidate for efficient transduction of epithelial cells, not restricted to the airway.